Gyan, 31, has been a regular for Ghana during the qualifying process, but an injury meant he did not turn out for his Turkish club Kayserispor’s 3-1 win over Bursaspor last weekend.
Midfielder Thomas Agyepong is also out injured, while Harrison Afful and Jonathan Mensah will have to undergo fitness tests in the United States after injuring themselves while playing for Major League Soccer side Columbus Crew.
Coach Kwesi Appiah has also left out Jordan and Andre Ayew from the squad, saying that he ‘was giving a chance to other players’.
There has however been speculation that the two are being punished for claiming to have been injured ahead of Ghana’s 5-1 win over Congo last month, only to turn out for their respective club sides in the English Premier League soon after.
Ghana lie third in Group E with five points; four behind leaders Egypt with two matches to play (the game away to Uganda, and a home clash with the Pharaohs on November 6).
Goalkeepers: Richard Ofori (Martizburg United, South Africa), Lawrence Ati (Sochaux, France), Joseph Addo (Aduana Stars)
Defenders: Harrison Afful (Columbus, USA), Daniel Opare (Augsburg, Germany) Agbenyenu Lumor (Portimonese, Portugal), Abass Mohammed (Harrisburg City, USA), Kasim Nuhu (Young Boys, Switzerland), Daniel Amartey (Leicester,England), Jonathan Mensah (Columbus Crew,USA), Nicholas Opoku (Club Africain, Tunisia), Vincent Atinga (Hearts of Oak)
Midfielders: Thomas Partey (Atletico Madrid, Spain), Joseph Attamah (Bakaksehir, Turkey), Christian Atsu (Newcastle United, England), Kingsley Sarfo (Malmo, Sweden), Alfred Duncan (Sassuolo FC, Italy), Ebenezer Ofori (Stuttgart, Germany), Frank Acheampong (Tianjin TEDA, China), Isaac Twum (Inter Allies), Patrick Twumasi (Astana FC, Kazakhtan)