The Three Lions top Group F on 20 points with six wins and two draws, five clear of second-placed Slovakia and six points better off than both Slovenia and Scotland.
Lithuania are in fifth position on five points, with Malta bottom with zero points.
Gareth Southgate’s side have been relatively convincing in recent matches, beating Malta 4-0 ahead of a 2-1 win over Slovakia, while Sreko Katanec’s team come into this match after a 1-0 loss to Slovakia and a 4-0 victory over Lithuania.
After announcing his squad recently, Southgate made some adjustments after some forced withdrawals.
One such change saw Tottenham midfielder Harry Winks drafted into the senior squad for the first time after Manchester City winger Fabian Delph picked up an injury.
Manchester United defender Phil Jones was also ruled out of contention by the FA’s medical staff.
It is a massive blow for Delph, who last played for his country in November 2015 having also missed Euro 2016 with a groin strain – but a huge opportunity for the England under 21 star Winks.
Spurs midfielder Dele Alli is, meanwhile, suspended for one game, following his offensive gesture in the 2-1 win over Slovakia, that was directed at Kyle Walker.
If England fail to defeat Slovenia, they can still secure qualification with points over Lithuania in Vilnius on Sunday.