Australia A were scheduled to play four-day games against their South Africa counterparts as well as limited-overs matches against the hosts and the India A side.
The players selected for the tour have agreed to convene for a training camp in Brisbane on Monday but will refuse to play matches if they do not sign new contracts.
All Australia’s leading players are currently unemployed after their deals with Cricket Australia expired at the end of last month, with the board and the players failing to agree terms on new contracts.
The Australia A players held an emergency meeting on Sunday which was also attended by national captain Steve Smith and agreed not to take part in any games unless new deals are signed by the end of the week.
Usman Khawaja, who was chosen to captain the A side in South Africa said: “It’s not an easy thing to do. Individually, I really want to play cricket. I haven’t played cricket for a long time. It’s the same with all the other guys.
“But we’re very united. It was quite an easy phone call in the end because everyone was going in the same direction.
“We’re still going to be training this week, going up there, doing our thing and getting ready. Hopefully something can be resolved but if it’s not it’s just a tough decision that sort of has to be made at this time.”
ACA Chief Executive Alistair Nicholson added: “The decision not to tour in the absence of a renewed MOU has been discussed and endorsed by the Australia A squad in solidarity.